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onsite at the Cell Center Stockroom with immediate access and no shipping costs
For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. NEB 5-alpha strains are enhanced DH5α™ derivatives, while NEB 10-beta is a DH10B™ derivative. NEB also offers a unique fast growing strain (NEB Turbo) and a dam–/dcm– strain.
NEB offers several strains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies:
SHuffle strains enable disulfide bond formation in the cytoplasm.
BL21 (DE3) is available for routine T7 expression in addition to Lemo21(DE3) for tunable expression of difficult targets such as membrane proteins, toxic proteins and proteins prone to insoluble expression.
Free of animal products
T1 phage resistance (fhuA2) Media and control plasmid included with most strains
A variety of convenient formats, including single use transformation tubes and, on request, 96 well plates